TABLE 1.
Mutations recovered after selection for intracellular invasion and/or intracellular cytotoxicity
| Common gene name | Reported virulence factor in S. aureus (reference)? | Protein accession no. | Gene function | Protein changea | Mutation type | Occurrence in selection isolates (n = 24) |
|
|---|---|---|---|---|---|---|---|
| Intracellular cytotoxicity | Intracellular invasion | ||||||
| pheT | No | AHM68762.1 | Phenylalanyl-tRNA synthase subunit beta | Leu110Ile | Missense | 0 | 1 |
| ctaB | Yes (56) | AHM68781.1 | Protoheme IX farnesyltransferase | Thr222Ile | Missense | 1 | 0 |
| qoxD | Yes (81, 83) | AHM68836.1 | Quinol oxidase subunit 4 | Gly83Arg | Missense | 0 | 2 |
| menD | Yes (77) | AHM68850.1 | 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase | Ile91fs | Frameshift | 0 | 1 |
| Ala83del | In-frame deletion | 6 | 0 | ||||
| menF | Yes (33) | AHM68851.1 | Isochorismate synthase | Ser31* | Stop gain | 0 | 1 |
| ndhC | No | AHM68945.1 | NADH dehydrogenase | Ala386Val | Missense | 0 | 1 |
| None (unnamedA) | No | AHM68983.1 | Hypothetical peptidase | Asp486Glu | Missense | 0 | 2 |
| saeS | Yes (72) | AHM69145.1 | Histidine kinase | Ala106Thr | Missense | 0 | 16 |
| nupC | No | AHM69205.1 | Nucleoside permease | Pro189Leu | Missense | 0 | 2 |
| mnhA | Yes (111) | AHM69226.1 | Monovalent cation/H+ antiporter subunit A | Ala722Thr | Missense | 0 | 2 |
| sdrC | Yes (84, 85) | AHM69286.1 | Hydrolase | Glu469Gly | Missense | 2 | 0 |
| tufA | No | AHM69299.1 | Elongation factor Tu | Ala271Val | Missense | 0 | 4 |
| purR | Yes (29, 30, 68) | AHM69351.1 | Purine operon repressor | Gly210fs | Frameshift | 6 | 0 |
| Ala208fs | Frameshift | 6 | 0 | ||||
| Ser189Leu | Missense | 0 | 4 | ||||
| Gly119Asp | Missense | 0 | 2 | ||||
| Gly67Arg | Missense | 0 | 2 | ||||
| Lys37Thr | Missense | 2 | 2 | ||||
| Val30fs | Frameshift | 0 | 2 | ||||
| epsC | No | AHM69661.1 | Polysaccharide biosynthesis protein | Asn333fs | Frameshift | 6 | 0 |
| ypfH | No | AHM69943.1 | Carboxylesterase | Ser104* | Stop gain | 0 | 3 |
| None (unnamedB) | No | AHM70189.1 | Hypothetical membrane protein | Phe41fs | Frameshift | 0 | 4 |
| murA | No | AHM70338.1 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | Ala378del | In-frame deletion | 4 | 0 |
| yhcF | No | AHM70475.1 | GntR family transcriptional regulator | Thr74Ile | Missense | 0 | 1 |
| aroE | No | AHM70790.1 | Shikimate dehydrogenase | Thr182Ile | Missense | 1 | 0 |
| Tyr164fs | Frameshift | 10 | 0 | ||||
| srrA | Yes (92) | AHM70893.1 | Transcriptional regulator | Arg197fs | Frameshift | 2 | 0 |
| Glu232* | Stop gain | 2 | 0 | ||||
| ubiE | No | AHM70914.1 | Ubiquinone biosynthesis methyltransferase | Lys21fs | Frameshift | 4 | 0 |
| ebh | Yes (78) | AHM70948.1 | Matrix-binding protein | Glu6622Lys | Missense | 0 | 4 |
| brnQ2 | Yes (112) | AHM70974.1 | Branched-chain amino acid ABC transporter | Asp198Tyr | Missense | 0 | 1 |
a
An asterisk indicates a stop gain mutation at the amino acid position listed.