FIG 2.

Four monoclonal antibodies derived from immunization with RII have minimal neutralization effects and map to F2 domain of RII-140. (A) Neutralization analysis of schizont-stage P. falciparum 3D7 and HB3 parasites cultured in the absence (PBS) or presence of 3 mg/ml control antibody (purple) or anti-RII antibody (blue; 1B10, 1G6, 4B7, or 4E7). Parasitemia was assessed after two cycles by microscopy and growth normalized to that of PBS-treated cultures. The experiment was done four independent times in triplicate, and all data are shown. Significant values were determined by one-way ANOVA with Dunnett’s multiple-comparison tests. (B) ELISA for MAbs 1B10, 1G6, 4E7, and 4B7 utilizing RII (black bars), F1 (orange bars), and F2 (blue bars) construct-coated plates.