Skip to main content
. 2019 Mar 25;87(4):e00716-18. doi: 10.1128/IAI.00716-18

FIG 6.

FIG 6

Mutations within the epitopes of MAbs result in loss of binding. (A) ELISA for MAbs 1E2, 9G3, 7E6, 9D3, 5G10, 2G10, and 3E3 utilizing RII wild-type (WT) and mutant construct-coated plates (residues mutated are listed in Table S3). 1E2, mutant 10, P = 0.0217; mutant 11, P ≤ 0.001; 9G3, mutant 11, P ≤ 0.001; 7E6, mutants 4 and 5, P ≤ 0.001; mutant 6, P = 0.0073; 5G10, mutants 7 and 14, P ≤ 0.001; 2G10, mutant 7, P = 0.0014; mutant 14, P ≤ 0.001; 3E3, mutants 7 and 14, P ≤ 0.001. (B) ELISA for MAbs 1B10, 1G6, 4E7, and 4B7 utilizing RII wild-type and mutant construct-coated plates (residues mutated are listed in Table S3). 1B10, no significant loss of binding; 1G6, 4B7, and 4E7, lane 16, P ≤ 0.001. (C) Residues substituted for each mutant mapped on the structure of RII. Sialyllactose is represented by green sticks.