FIG 3.

SasG is a DNA binding protein. (A) DNase I sensitivities of the biofilms formed by the indicated strains. Relative biofilm biomasses are shown (nontreated biofilms are defined as 100%). Original data are shown in Fig. S1 in the supplemental material. −, absence; +, presence. (B) The DNA binding capacity of SasG was analyzed by a gel shift assay. Purified SasG (0.1, 0.5, and 1 μM) was mixed with lambda DNA (λDNA) prior to agarose gel electrophoresis. (C) Degradation of λDNA by DNase I was analyzed in the presence and absence of purified SasG. After treatment, DNase I and SasG were degraded by proteinase K, and the residual DNA was analyzed by agarose gel electrophoresis. Band intensities were measured using an LAS-4000 image analyzer, and the relative intensities are shown in the graph.