FIG 4.
The LPXTG motif in SasG plays a role in compensating for the loss of Eap in biofilm formation. (A) The domain structure of MR23 SasG. S, signal peptide; A, A domain; B, B domain; LPXTG, LPXTG motif. Plasmids for the expression of SasGWT and SasGΔL were constructed in the present study (see Table S1 in the supplemental material). (B) Biomasses of biofilms produced by the indicated strains were determined as described in the legend of Fig. 1. The means and standard deviations from three independent experiments are shown. **, P < 0.01. (C and D) SasG proteins in the cell wall fractions (C) and culture supernatants (D) were analyzed by Western blotting using anti-SasG antibody.