FIG 1.

Shigella demonstrates basolateral invasion preference in human colon enteroid monolayers. (A) S. flexneri WT and CFS100 strains were introduced apically or basolaterally to colon monolayers grown on transwells, and bacteria were enumerated at 3 h p.i. Statistical significance was determined by ANOVA with a Bonferroni posttest (n = 9; *, P < 0.05), with data pooled from three independent experiments. (B) Representative Giemsa stain of colonoid monolayers infected apically or basolaterally with WT S. flexneri or the noninvasive CFS100 strain.