FIG 2.

Shigella invades human enteroid monolayers derived from different segments. S. flexneri WT and CFS100 strains were introduced basolaterally to enteroid monolayers grown on transwells, and bacteria were enumerated at 3 h p.i. WT S. flexneri recovery was significantly higher than CFS100 recovery in all cell lines, as determined by ANOVA with a Bonferroni posttest (data from two independent experiments were pooled; n = 6; *, P < 0.05), while no statistically significant differences were observed among enteroid segment lines (P > 0.05 by ANOVA with a Bonferroni posttest).