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Shigella replicates within human colon enteroid monolayers. S. flexneri WT strain was introduced basolaterally to enteroid monolayers grown on transwells, and bacteria were enumerated over a time course. Line represents a nonlinear regression using the exponential growth model (R2, 0.80). The mean doubling time is calculated to be 47.1 ± 6.7 min, from two independent experiments.
