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Shigella infection alters host gene expression. Colon enteroid monolayers were treated with EGTA, S. flexneri WT or CFS100 bacteria were then introduced apically, and host gene expression was quantified at 3 h p.i. using RT-qPCR. Gene expression was normalized to that of actB, and log2-fold change values are relative to those of the mock-infected control. Statistical significance was determined by a t test, comparing ΔCT values (n = 3; *, P < 0.05).
