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. 2019 Mar 25;9:5052. doi: 10.1038/s41598-019-41513-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased Antifungal Tolerance of 10 Generation Old C. auris cells from FLC- Resistant S9 strain: (a) qPCR analysis to study the expression of CDR1 and ERG11 in young and old cells. ACT1 gene was used as an internal control. The data was normalized to the gene expression in young cells. The assay was done in biological triplicate and error bars signify standard deviation. Multiple t-test was performed using Holm-Sidak method to analyze the significance; *p < 0.05. (b) R6G efflux assay performed on young (black line) and old (red line) in triplicate and error bars signify standard deviation. Student’s t-test with Welch’s correction was performed to determine the significance; *p < 0.05.