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. 2019 Mar 25;9:5052. doi: 10.1038/s41598-019-41513-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR Analysis to Study the Increase in Copy Numbers of CDR1 and ERG11 in Old C. auris cells isolated from strain S1.: (a) Cartoon showing the location of the oligonucleotides used in this analysis. The primers span 3 different regions of each genes. “P” denotes primer. All primers listed in Table S1. Genomic DNA was isolated from both old (10 generation) cells and young (0–3 generation) cells of FLC-sensitive strain S1 (b) and FLC-resistant strain S9 (c). Data shows the gene copy numbers of CDR1 and ERG11 using oligo sets P7-P8 (CDR1) and P5-P6 (ERG11) ACT1 was used as a control for the experiment and the data was normalized to the gene copy number of young cells. The assay was performed in triplicate and error bars signify standard deviation. Multiple t-test was performed using Holm-Sidak method to analyze the significance; *p < 0.05.