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. 2019 Mar 25;9:5052. doi: 10.1038/s41598-019-41513-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FLC Treatment of Galleria infected with Young (0–3 Gen) and Old (10 Gen) cells of C. auris: 20 larvae were infected separately with 10⁵ (a) young and (b) old cells. 1 µg/ml of FLC was injected to the larvae infected with young and old population on the same day of infection. Log rank test was used to determine the statistical significance. (c) Cells from old (10 Gen) S1 strain were significantly retained more in the haemolymph (*p < 0.05) when compared to young cells at different time points. Haemolymph was collected from 15 larvae over the period of infections (0, 0.5, 2, 4, 8 and 24 h) as described and plated in YPD plates. CFUs were counted and plotted after 48 h of incubation at 37 °C. For each time point, 5 × 10⁴ young and old cells from isolate S1 was infected into 15 larvae. Error bars represent standard deviation in the CFU counts. (n = 15) for each infection time point. Multiple t-test was performed using Holm-Sidak method to determine the statistical significance (*p < 0.05).