Table 1.
Genes | Names | Primers (5′–3′) |
---|---|---|
Nestin | Nestin-F | CTGCTACCCTTGAGACACCTG |
Nestin-R | GGGCTCTGATCTCTGCATCTAC | |
NSE | NSE-F | AGCCTCTACGGGCATCTATGA |
NSE-R | TTCTCAGTCCCATCCAACTCC | |
GFAP | GFAP-F | AGGTCCATGTGGAGCTTGAC |
GFAP-R | GCCATTGCCTCATACTGCGT | |
GAPDH | GAPDH-F | ACAACTTTGGTATCGTGGAAGG |
GAPDH-R | GCCATCACGCCACAGTTTC |
Primers were designed using Primer Express version 2.0 software. Primer specificity was confirmed using Primer-BLAST web software (National Centre for Biotechnology Information).