Figure 3.

Caspase‐3 activation is required for nuclear condensation and histone release in human terminal erythropoiesis. A, The effect of caspase‐3 inhibitor on nuclear condensation. CD34+ cultured in Epo‐containing medium were treated with caspase‐3 inhibitor (Caspase‐3i) on day 3 in culture and collected on day 7 or day 10. Nuclear size was measured by calculating the DAPI‐positive area using the ImageJ software. Each dot represents a single cell. Data were obtained from three independent experiments. B, Western blot analysis of indicated proteins from fractionated cytoplasmic (C) and nuclear (N) lysates of indicated cells. CD34+ cells were treated with caspase‐3/7 inhibitor (casp‐3/7i) or pan‐caspase‐3 inhibitor (pan‐casp‐3i) on day 3 in culture and collected on day 7 or day 10. An equal number of cells were loaded in each lane