Figure 4.

CD9 is not expressed on normal CD34+CD38− physiologic cells but is expressed on phenotypically most immature AML cells A. Blast cells are separated using CD34 and CD38 expression into several progenitors:P6 (CD34+CD38−), P7 (CD34+CD38dim) and P8 (CD34+CD38+) cell compartments B. Determination of HSC, MPP, LMPP in a normal bone marrow sample C. CD9 is not expressed on HSC D. Blast cells are separated using CD34 and CD38 expression into P6, P7, and P8 cell compartments E. Determination of HSC, MPP, and putative LSC (CD34+CD38−CD90dimCD45RA+) in a bone marrow sample from a patient at AML diagnosis F. CD9 is expressed on putative LSCs at similar MFI level compared to AML blast cells