Figure 4.

HIF-1α combined with Bio-Oss upregulates the expression of Runx2 and OCN in vivo. Immunohistochemistry staining with (A) anti-Runx2 antibody, (B) anti-OCN antibody and (C) anti-HIF-1α antibody of paraffin sections from bones in the extraction sites. (D) Quantification of Runx2-positive stained cells in (A). (E) Quantification of OCN-positive stained cells in (B). (F) Quantification of HIF-1α-positive stained cells in (C). Data were presented as mean ± standard deviation, n>3. *P<0.05, **P<0.01 as indicated. HIF-1α, hypoxia-inducible factor-1α; Runx2, runt-related transcription factor 2; OCN, osteocalcin.