Table I.
Gene | Primer sequences (5′-3′) |
---|---|
Runx2 | F: TCCAGACCAGCAGCACTCCATA |
R: TTCCATCAGCGTCAACACCATC | |
ALP | F: CTGCGGGACTCAACGACACT |
R: AGGAGGGTACTCATTGGC ATAGC | |
OCN | F: TCACAGACCCAGACAGAACCG |
R: AGCCCAGAGTCCAGGTAGCG | |
HIF-1α | F: GTGTACCCTAACTAGCCGAGGA |
R: GTTCACAAATCAGCACCAAGC | |
VEGF | F: AAGAGCGATCCCCACGTCAA |
R: TTCGTTTCAGTGCCACATACCA | |
GAPDH | F: ATGTTTGTGATGGGCGTGAAGGTC |
R: TTCTGGGTGGCAGTGAT |
F, forward; R, reverse; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.