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Primer sequences in RT-PCR.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Human GAPDH | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |
| Human IL-1β | CTGAGCACCTTCTTTCCCTTCA | TGGACCAGACATCACCAAGCT |
| Human IL-6 | TGGCTGAAAAAGATGGATGCT | TCTGCACAGCTCTGGCTTGT |
| Human TNF-α | TGTAGCCCATGTTGTAGCAAACC | GAGGACCTGGGAGTAGATGAGGTA |
RT-PCR, reverse transcription-polymerase chain reaction; GAPDH, glyceraldehyde-phosphate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor.
