Fig. 3.

Regulation of USP expression by DMOG and TGF-β in fibroblast cell lines

Synovial fibroblasts (n = 6 cell lines), obtained from the knee of patients with OA, were cultured for 24 h in duplicate wells with DMOG, a hypoxia-inducible factor 1α hydroylase inhibitor, or TGF-β. Expression of the indicated USP was evaluated by quantitative PCR. Results are displayed as mean (± s.e.m.) fold-changes compared with unstimulated cells from three different experiments. *P < 0.01; **P < 0.005; ***P < 0.0001 using unpaired Student’s t-tests. Mean value for USP37 induction by DMOG, 19.1. DMOG: dimethyloxalylglycine; ND: not done; USP: ubiquitin-specific peptidase.