Table 1. The primer sequences for RT-qPCR.
Gene | Primer sequence (5′–3′) |
---|---|
Apc | Forward: CTTCGTGTACGGCAGCTCTT |
Reverse: GCAGTTTCATGCTTGCTCTG | |
NF-κB p65 | Forward: GAAGAAGCGACCTGGAG |
Reverse: TCCGGAACACAATGGCCAC | |
IκBα | Forward: CCACGACAGCGGCTTGGACTA |
Reverse: TCCACGATGCCCAGGTAGCC | |
MMP-2 | Forward: CTGATAACCTGGATGCAGTCGT |
Reverse: CCAGCCAGTCCGATTTGA | |
MMP-9 | Forward: TTCAAGGACGGTCGGTATT |
Reverse: CTCGAGCCTAGACCCAACTTA | |
TNF-α | Forward: TGAGCACAGAAAGCATGATC |
Reverse: CATCTGCTGGTACCACCAGTT | |
IL-1β | Forward: GACCTGTTCTTTGAGGCTGAC |
Reverse: TCCATCTTCTTCTTTGGGTATTGTT | |
IL-6 | Forward: GACTGATGTTGTTGAGAGCCACTG |
Reverse: TAGCCACGCCTTCTGTGACTCTAACT | |
GAPDH | Forward: CATCAACGACCCCTTCATTG |
Reverse: GAAGATGGTGATGGGTTTCC |
Abbreviation: MMP, matrix metalloproteinase.