Figure 4.

In vivo myocardial superoxide and nitrotyrosine levels. Myocardial (A) superoxide by chemiluminescence, (B) DHE staining and quantitative analysis, (C) nitrotyrosine by ELISA, and (D) nitrotyrosine immunoreactive staining and quantitative analysis from the border zone. Myocardial DHE (red fluorescent) and nitrotyrosine (brown) staining showed more intense signals (nuclear position for DHE and cytoplasm for nitrotyrosine) after MI. Compared with vehicle, myocardial superoxide and nitrotyrosine levels in the OM-treated infarcted group were significantly increased, which can be significantly reduced after adding either Vit C or ARB. Bar = 50 μm. Sham (n = 10); Vehicle (n = 10); OM, omeprazole (n = 11); OM/Vit C, vitamin C (n = 11); OM/ARB, olmesartan (n = 12); FM, famotidine (n =10). * p < 0.05, compared with sham; ^† p < 0.05, compared with vehicle-treated rats; ^‡ p < 0.05, compared with OM-treated rats. Abbreviations as in Table 1