Figure 5.

Myocardial DDAH and eNOS activity assessment. (A) Myocardial DDAH activity, (B) DDAH2 Western blot, (C) DDAH2 RT-PCR, and (D) eNOS activity from the border zone. Compared with vehicle in omeprazole-treated rats, DDAH activity, protein level and mRNA expression were significantly decreased, which can be reversed by adding vitamin C. When compared with vehicle-treated infarcted rats, omeprazole-treated infarcted rats had significantly lower p-eNOS levels. Relative abundance was obtained by normalizing the density of eNOS protein against that of β-actin. Sham (n = 10); Vehicle (n = 10); OM, omeprazole (n = 11); OM/Vit C, vitamin C (n = 11); OM/ARB, olmesartan (n = 12); FM, famotidine (n =10). * p < 0.05, compared with sham; ^† p < 0.05, compared with vehicle-treated rats; ^‡ p < 0.05, compared with OM-treated rats. Abbreviations as in Table 1. DDAH, dimethylarginine dimethylaminohydrolase; RT-PCR, real-time polymerase chain reaction.