Ex vivo myocardial superoxide and DDAH2 levels. (A) Myocardial superoxide levels assessed by DHE staining and (B) DDAH2 protein levels from the border zone. Compared with vehicle, myocardial superoxide and DDAH2 levels in the omeprazole-treated infarcted group were not significantly changed, which contrasted with the in vivo study. Bar = 50 μm. Vehicle (n = 5); OM, omeprazole (n = 5); FM, famotidine (n = 5); OM/Vit C, vitamin C (n = 5). * p < 0.05, compared with vehicle-, omeprazole- and famotidine-treated rats. Abbreviations as in Table 1