Fig. 4.

(a) Representative western blots and bar graphs showing the expression of cav-1 in controls (C) and hypoxic (H) neonatal calves (n = 5–6). (b) Immunofluorescence studies also did not show any alterations in the endothelial cav-1 expression in the neonatal hypoxia group (b, H1–H4) or in steers with Brisket disease (c, B1–B4). There is significant medial thickening. In H1, H2, and H3, few green speckles are seen in the medial layer. SMC normally has cav-1 which often is not well seen in immunofluorescence studies, but occasionally one can see a few speckles. It is important to note that the western blot does not reveal increased cav-1 expression in these bovine lung tissues. Cav-1 (green color) and SM α-actin (red). C, control; H, hypoxia; H1–H4, hypoxia groups; B1–B4, Brisket disease groups.