Figure 3.
(a) 1H-[13C]-NMR spectrum depicting 13C labeling of hippocampal amino acids from [2-13C]acetate in AβPP-PS1 mice. Mice were infused with [2-13C]acetate for 15 min. Metabolites were extracted from frozen hippocampal tissue. 1H-[13C]-NMR spectra were recorded in hippocampal extracts. (b) The cerebral metabolic rate of acetate oxidation (CMRAc(ox)) in AβPP-PS1 and control mice. The 13C labeling of brain amino acids were measured using 1H-[13C]-NMR spectroscopy in tissue extracts. The CMRAc(ox) was calculated using equation (1). *p < 0.5 when compared with the respective controls.