Assessment of outcomes and brain atrophy with Cresyl violet staining
after treatment. (a) Rats treated with young human mesenchymal stem
cells (hMSCs) (n = 9) showed better behavioural
recovery by modified neurological severity score than controls
(n = 10) or old hMSCs (n = 8)
(Steel–Dwass test, *P < 0.05 vs. Control,
#P < 0.05 vs. Old hMSC). Each
point represents the median (interquartile range, 25–75th
percentile) in the graph. (b) Cresyl violet staining. Scale
bar = 1 mm. (c) Left ventricle size in young hMSCs was significantly
smaller than controls or old hMSCs (one-way ANOVA, Tukey–Kramer
multiple comparison test, *P < 0.05). Data are
presented as mean ± SD. (d) There were no significant differences in
animal survival rates between the three groups
(n = 26 in young hMSC group,
n = 25 in old hMSC group and
n = 19 in controls; Log-rank test).