Figure 2.

Cell culture protocol timelines. (A) Human SMCs were seeded onto the bone scaffold—either DB or HB—and maintained in SMC medium for 3 weeks. Afterwards, HUVECs were seeded, and the constructs cultured in EC medium for 6 days. Fibrin hydrogel was finally added, with ongoing culture in EC medium, for 1–5 days. (B) HUVEC-only culture timeline. DB scaffold was pre-treated with fibronectin for 1 h, and then only HUVECs were seeded and cultured for 6 days, followed by application of fibrin sealant for 1–5 days. Red arrows indicate the work flow direction.