Skip to main content
. 2018 Jul 19;27(8):1222–1234. doi: 10.1177/0963689718779361

Figure 8.

Figure 8.

H2S regulates the expression of LC3-II, Beclin1, and P62 during autophagy. (a) Western blot analysis of HCF-av cells upon different treatments was performed to detect LC3I/II, Beclin 1, and P62. (b–d) Quantitative analysis of the changes of LC3I/II, Beclin1, and P62 in treated cells (n = 3). *P < 0.01 vs. control; *P < 0.01 vs. NaHS; #P < 0.05 vs. H2O2. (e) Proteasome activity was measured using AMC-linked substrate peptides under different treatments (n = 3). (f) Representative Western blot analysis for ubiquitin expression. β-actin served as the loading control. (g) Quantitative analysis of the changes of ubiquitin in treated cells (n = 3).