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. 2018 Mar 22;27(1):55–69. doi: 10.1177/0963689717723016

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 2. — Exosomes from Refeed^®-supplemented hFM-MSCs enhance their migration ability without modifying vasculogenic properties. (A) Representative images (4× magnification) of the whole wound area in the scratch assay taken at 0 (upper panel) and 16 h (lower panel) after cell monolayer scratching and concurrent supplementation of vehicle or exosomes. Control cells treated Fig. 2 (continued). with vehicle (CV), with exosomes derived from control hFM-MSCs (CC), or with exosomes derived from Refeed^®-supplemented hFM-MSCs (CR); Refeed^®-supplemented cells treated with vehicle (RV), with exosomes derived from control hFM-MSCs (RC), or with exosomes derived from Refeed^®-supplemented hFM-MSCs (RR). (B) The migration rate of control cells supplemented with exosomes derived from Refeed^®-supplemented hFM-MSCs (CR and RR) was significantly higher compared with exosomes derived from control hFM-MSCs (CC and RC) or with vehicle (CV and RV). The migration rate is represented as percentage of scratch closure area. *Significant difference between two groups. Each bar represents the mean ± SD (n = 3, one-way analysis of variance with subsequent Tukey’s test, *p < .05). (C) In vitro capillarogenesis assessed in control (C) and Refeed^®-supplemented (R) hFM-MSCs exposed to exosomes derived from control (CC or RC) or Refeed^®-supplemented (CR or RR) cells. Representative images of total well area in tube formation assays after 12 h. (D) Bar graph showing quantification of tube formation at 12 h in CC, CR, RC, and RR cells. Each bar represents the mean ± SD (n = 6; one-way analysis of variance with subsequent Tukey’s test).