Table 1.

Native MSC Yields from Umbilical Cord Tissue (UCT) Processed with the AuxoCell Processing (AC:Px) System (Both in the Absence and Presence of Collagenase) and Manually Processing in the Presence of Collagenase Were Determined.

		Live Native MSCs per gram
AC:Px®	Collagenase	Average	SEM	N
−	+	2.65 × 105	0.45 × 105	31
+	−	7.20 × 105	0.53 × 105	31
+	+	3.79 × 105	0.58 × 105	6

Note: Umbilical cord tissue (UCT) was cut into equivalent halves and manually minced with umbilical cord scissors for collagenase treatment. Meanwhile, the other UCT half was placed in its entirety into the AC:Px Mincer for mechanical mincing, without the need or use of collagenase. In order to further understand collagenase effects on recovered UCT native cells, the native cells and minced tissue products from the AC:Px System from several units were further processed in the presence of collagenase. The average cell yields shown are the number of live native mesenchymal stem/stromal cells (MSCs) recovered per gram of processed UCT ± the standard error of the mean (SEM). Live native MSCs represent 7AAD⁻, CD45⁻, CD29⁺, and CD90⁺ cells in each of the recovered populations.

Live native MSCs = 7AAD⁻/CD45⁻/CD29⁺/CD90⁺.