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. 2018 Mar 22;27(1):130–139. doi: 10.1177/0963689717728937

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Assessment of FVIII expression by transduced PMSCs. RT-PCR (A) and Western blot (B) analysis of expression of BDD-FVIII by transduced PMSCs (A: lane 2 and B: lane 2, respectively), compared to PMSCs transduced with control vector (A: lane 1 and B: lane 1, respectively). Comparison of human FVIII secretion by ELISA (C) and activity by chromogenic assay (D) between PMSCs transduced with BDD-FVIII vector and PMSCs transduced with control vector. n = 4 pairs of PMSC cell lines. PMSC, placenta-derived mesenchymal stromal cell; BDD-FVIII, B-domain-deleted factor VIII; FVIII, factor VIII; RT-PCR, reverse transcription PCR; ELISA, enzyme-linked immunosorbent assay.