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. Author manuscript; available in PMC: 2019 Mar 26.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2018 May 3;1864(9 Pt B):2753–2760. doi: 10.1016/j.bbadis.2018.04.023

Figure 3: The abundance of exon 10 containing circ RNA is regulated by the kinase clk2.

Figure 3:

One μg of the tau exon 9–12 minigene was transfected into HEK293 cells together with 1 μg of the plasmids expressing the trans-acting factors indicated.A. Linear RNA was amplified using primers in exon 9 and 11, B. Circular RNA was amplified using primers rev12–10 and forward_exon 10. C. The circular HIPK3 RNA was amplified as a loading control, D. Quantification of the circtau to circHIPK3 ratio, E. Quantification of a cotransfection of the SR-proteins tra2-beta1 and 9G8 in the presence of their kinases CLK2 and SRPK1. 500 ng of each expressing vectors were cotransfected and three independent experiments were analyzed.