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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Feb 16;511(2):280–286. doi: 10.1016/j.bbrc.2019.02.021

Fig. 2.

Fig. 2.

Oral epithelial sheets from the palate at low (A) and high (B) magnification, and from the base of the oral cavity at low (C) and high magnification (D). The bright-field image, distribution of Vimentin (red) and T1R1 (green) immunosignals, and merged images of oral epithelial sheets of chickens are shown. The merged image shows the overlay of Vimentin (red) and T1R1 (green). Arrowheads shown in B indicate representative taste bud clusters characterized by the Vimentin signal or by the morphology from the bright-field image. Scale bars: 500 μm in (A,C) and 100 μm in (B,D). Quantitative analyses of the numbers of Vimentin-expressing (Vimentin+) bud clusters and T2R7-expressing (T2R7+) bud clusters in the ppr (E) or the numbers of Vimentin+ bud clusters and T1R1-expressing (T1R1+) bud clusters in the ppr (F) are represented as means ± standard error (SE) (n = 3–5). There were no significant differences between the numbers of Vimentin+ bud clusters and T2R7+ bud clusters (P > 0.05) (E), or between the numbers of Vimentin+ bud clusters and T1R1+ bud clusters (P > 0.05) by paired Student t-test (F).