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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Sep;38(9):2191–2197. doi: 10.1161/ATVBAHA.118.310870

Figure 1.

Figure 1.

Generation of smooth muscle-like cells (iSMCs) from MEFs. (A) Schematic drawing of the differentiation protocol used to generate iSMCs. (B) Induction of expression of the smooth muscle cell marker Myh11 in MEFs treated with a combination of Myocd*, Gata4, Mef2B*, and Mef2C* genes on day 5 post-transduction. (C) Induction of Myh11gene expression in MEFs treated with combination of Myocd*, Mef2C*, Gata4, and Gata6 genes and cultured for the 3, 4 and 5 days post-transduction. (D) Downregulation of expression of fibroblast marker genes Fn-EDA, Thy1 in MEFs at 5 days post-transduction with the retrovirus encoding the reprogramming factors indicated in the figure. Normalized with empty vector virus control group = 1.0 (B and C) or Myocd*, Mef2C*, and Gata6 group = 1.0 (D). The data are shown as mean ± SEM. N=3. ****p < 0.001, ***p < 0.01, **p < 0.05 compared with the control group. G4: Gata4, G5: Gata5, G6: Gata6, Md: Myocd, Md*: Myocd*