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. Author manuscript; available in PMC: 2019 Mar 26.
Published in final edited form as: Mol Cell. 2018 Sep 27;72(2):355–368.e4. doi: 10.1016/j.molcel.2018.08.021

Figure 1. RIG-I activation pathway and RIG-I/RD on-rates on 5’ppp and non-PAMP dsRNAs.

Figure 1.

(A) Schematic of a general activation mechanism of RIG-I. (B) Cartoon of the dsRNAs used in the study (green asterisk-DY547/Cy3; blue square-biotin; blue bars-DNA bases). (C) IFN-β reporter activation in HEK293T cells expressing RIG-I and transfected with the dsRNA panel from B. Error bars are SEM from quadruplicate sets. (D) Stopped-flow experimental set up for on-rate and off-rate measurements. (E) Representative stopped-flow time course shows an increase in fluorescence intensity upon binding of RIG-I (45 nM) to 5’ppp dsRNA (10 nM) at 25°C. (F, G) On-rates of RIG-I and RD. The associated errors from the linear fits are shown. See also Figure S1.