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. Author manuscript; available in PMC: 2019 Mar 26.
Published in final edited form as: Mol Cell. 2018 Sep 27;72(2):355–368.e4. doi: 10.1016/j.molcel.2018.08.021

Figure 2. Off-rates of 5’ppp and non-PAMP RNAs from RD, Helicase-RD, and RIG-I.

Figure 2.

(A-C) Stopped-flow kinetics of RNA dissociation. A preformed complex of RD (A), Helicase-RD (B), or RIG-I (C) with the specified fluorophore-tagged RNA was chased with a 10-fold excess of unlabeled 5’ppp ds12 hairpin RNA at 25°C. (D) RIG-I forms two distinct complexes with dsRNA: RD-mode and Helicase-mode conformations. (E-F) Lifetimes of RD-mode (E) and Helicase-mode (F) RIG-I complexes calculated from reciprocal of corresponding off-rates. Representative kinetic traces are an average of 4–6 traces. See also Figure S2 and Table S1.