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. Author manuscript; available in PMC: 2019 Mar 26.
Published in final edited form as: Mol Cell. 2018 Sep 27;72(2):355–368.e4. doi: 10.1016/j.molcel.2018.08.021

Figure 3. Off-rates of 5’ppp and non-PAMP RNAs in the presence of ADP.BeF3 and ATP.

Figure 3.

(A) Representative off-rate kinetics of RIG-I from 5’ppp dsRNA. (B) Lifetimes of the RD-mode population. (*) indicates that an RD-mode population was not observed. Relative percentages (C) and lifetimes (D) of the Helicase-mode population of RIG-I. (E) Helicase-mode lifetimes of RIG-I:RNA complexes in the presence of the specified ATP analog (F) Kd values of RIG-I:RNA complexes normalized to the 5’ppp dsRNA:RIG-I complex. (G) MANT-ATP Kd values. (H-I) Lifetimes of the Helicase-mode population of E373A (H) and C268F RIG-I (I). Representative kinetic traces are an average of 4–6 traces. See also Figure S3 and Tables S2–S6.