FIG. 4.

Shortening the ex vivo challenge assay with one-step ddPCR compared to p24. (A) Pattern of cumulative one-step ddPCR with shortened explant windows. Cumulative values were calculated by taking the sum of days 3 through 14 (open blue squares for ddPCR and solid red circles for p24), days 3 through 10 (solid orange triangles; ddPCR only), and days 3 through 7 (open purple circles; ddPCR only) of explant culture, adjusted for biopsy weight, and log transformed. Each symbol represents the mean ± SE at each study visit (n = 24; 6 patients each with 4 biopsy replicates). (B) Linear relationship between cumulative p24 (days 3–14) versus cumulative one-step ddPCR days 3–10 only or versus (C) one-step ddPCR days 3–7 only. Units and statistical test for (A) are the same as those presented in Figure 2.