Table 1.
Number of HIV-1 RNA or p24 Positive Samples for Each Day of Collection in the Ex Vivo Challenge Assay Determined by ddPCR and ELISA
| Baseline positive/total (%) | Day 28 positive/total (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| Assay | Day 3 | Day 7 | Day 10 | Day 14 | Day 3 | Day 7 | Day 10 | Day 14 |
| One-step ddPCR | 23/24 (96) | 21/24 (88) | 21/24 (88) | 20/24 (83) | 20/23 (87) | 22/24 (92) | 14/24 (58) | 9/22 (41) |
| One-step automated ddPCR | 21/24 (88) | 22/24 (92) | 22/24 (92) | 21/24 (88) | 22/24 (92) | 19/24 (79) | 11/24 (46) | 7/22 (32) |
| Two-step ddPCR | 22/24 (92) | 20/24 (83) | 18/24 (75) | 18/24 (75) | 13/23 (57) | 7/24 (29) | 5/24 (21) | 3/22 (14) |
| HIV-1 p24 ELISA | 0/24 (0) | 9/24 (38) | 17/24 (71) | 18/24 (75) | 1/23 (4) | 0/24 (0) | 1/24 (4) | 1/22 (5) |
ddPCR, droplet digital PCR; ELISA, enzyme-linked immunosorbent assay.