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. 2019 Mar 26;17:43. doi: 10.1186/s12951-019-0476-0

Table 1.

Primers and probes used in this study

Primer name Sequence (5′ → 3′) Target Size Reference
PCR F: TTGAAGGTAATTTAGATATG
R: CTAATACCTAAAGTTGAAAC
C. jejuni/C. coli 400 bp [49]
Duplex-PCR FU: TTGAAGGTAATTTAGATATG
R1: TTTATTAACTACTTCTTTTG
R2: ATATTTTTCAAGTTCATTAG
C. coli
C. jejuni
461 bp
737 bp
[10, 49]
Real-time PCR F: AACCCAAATTCTAATTGATC
R: GAAGGTAATTTAGATATGGATAA
P: FAM-AAATGATAACCAAGTCTAATCCCTGG
C. jejuni/C. coli 95 bp This study
Biosensor Probe 1: TTATCCATATCTAAATTACC-SH
Probe 2: SH-AGTCTAATCCCTGGTGCATA
C. jejuni/C. coli 44 bp This study
Cloning M13F: AGGGTTTTCCCAGTCACGA
M13R: GAGCGGATAACAATTTCACAC
Recombinant plasmid (for sequencing) 193 bp [50]