Table 1.
Primer name | Sequence (5′ → 3′) | Target | Size | Reference |
---|---|---|---|---|
PCR | F: TTGAAGGTAATTTAGATATG R: CTAATACCTAAAGTTGAAAC |
C. jejuni/C. coli | 400 bp | [49] |
Duplex-PCR | FU: TTGAAGGTAATTTAGATATG R1: TTTATTAACTACTTCTTTTG R2: ATATTTTTCAAGTTCATTAG |
C. coli C. jejuni |
461 bp 737 bp |
[10, 49] |
Real-time PCR | F: AACCCAAATTCTAATTGATC R: GAAGGTAATTTAGATATGGATAA P: FAM-AAATGATAACCAAGTCTAATCCCTGG |
C. jejuni/C. coli | 95 bp | This study |
Biosensor | Probe 1: TTATCCATATCTAAATTACC-SH Probe 2: SH-AGTCTAATCCCTGGTGCATA |
C. jejuni/C. coli | 44 bp | This study |
Cloning | M13F: AGGGTTTTCCCAGTCACGA M13R: GAGCGGATAACAATTTCACAC |
Recombinant plasmid (for sequencing) | 193 bp | [50] |