Skip to main content
. Author manuscript; available in PMC: 2019 Mar 26.
Published in final edited form as: Pharmacol Ther. 2016 Aug 7;171:93–103. doi: 10.1016/j.pharmthera.2016.08.003

Fig. 2.

Fig. 2.

Human pericytes and adventitial cells undergo roughly similar osteogenic differentiation. (A,B) Human pericytes and adventitial cells from the same patient sample were cultured under osteogenic conditions (10% FBS, 100 μg/ml ascorbic acid, 10 mM β-glycerophosphate). (A) Representative image of alkaline phosphatase (ALP) staining at 5 d differentiation. (B) Semi-quantification of ALP staining. (C,D) Human pericytes or adventitial cells were implanted in the thigh complex of a SCID mouse using a collagen sponge carrier (2.5 × 105 cells, sponge size 2.0 × 1.0 × 0.5 cm). (C) 3-Dimensional MicroCT reconstructions. (D) MicroCT based quantification of bone mineral density (BMD) and bone volume (BV).