. 2019 Mar 26;12:122. doi: 10.1186/s13071-019-3389-8

Table 1.

Targeted organisms and list of primers used in this study

Target organism	Primer	Sequence (5′–3′)	Fragment length (bp)	Reference
Babesia spp. & Hepatozoon spp.	Piroplasmid-F	CCAGCAGCCGCGGTAATTCCTTTCGC	400	[6]
Babesia spp. & Hepatozoon spp.	Piroplasmid-R	AGTAGTTYGTCTTTAACAAATCT	400	[6]
Babesia spp.	Babesia18S-F	CCGTGCTAATTGTAGGGCTAATACA	551	[7]
Babesia spp.	Babesia18S-R	GCTTGAAACACTCTARTTTTCTCAAAG	551	[7]
Babesia spp.	455-479F^a	GTCTTGTAATTGGAATGATGGTGAC	340	[8]
Babesia spp.	793-772R^b	ATGCCCCCAACCGTTCCTATTA	340	[8]
Babesia gibsoni	BgibAsia-F^c	ACTCGGCTACTTGCCTTGTC	185	[8]
Babesia vogeli	BCV-F^d	GTTCGAGTTTGCCATTCGTT	192	[8]
Hepatozoon spp.	Hepatozoon18S-F	GGTAATTCTAGAGCTAATACATGAGC	574	[7]
Hepatozoon spp.	Hepatozoon18S-R	ACAATAAAGTAAAAAACAYTTCAAAG	574	[7]
Ehrlichia spp.	E.c 16S-fwd	TCGCTATTAGATGAGCCTACGT	123	[3]
Anaplasma spp.	E.c 16S-rev	GAGTCTGGACCGTATCTCAGT	123	[3]
Anaplasmataceae	ECB	CGTATTACCGCGGCTGCTGGCA	500	[4]
Anaplasmataceae	ECC	AGAACGAACGCTGGCGGCAAGC	500	[4]
Ehrlichia canis	ECAN5	CAATTATTTATAGCCTCTGGCTATAGGA	400	[4]
Ehrlichia canis	HE3	ATAGGGAAGATAATGACGGTACCTATA	400	[4]
Anaplasma platys	ApysF	GTCGAACGGATTTTTGTCGT	200	[4]
Anaplasma platys	Apys	RTAGATCACCGCCTTGGTAGG	200	[4]
Filariid worms	Forward	TTTAAACCGAAAAAATATTGACTGAC	115	[5]
Filariid worms	Reverse	AAAAACTAAACAATCATACATGTGCC	115	[5]

^aNested PCR outer forward primer

^bNested PCR outer reverse primer

^cNested PCR inner reverse primer

^dNested PCR inner reverse primer