Table 3.
Concentrations of IFN-γ and level of positivity to L. infantum tests according to results of tests and the clinical status in IFN-γ producer and non-producer cats
| L. infantum tests or clinical status | No. of cats (%) | LSA IFN-γ (pg/ml)a |
ConA IFN-γ (pg/ml)a |
ELISA (EU)a |
IFAT (titer)a |
PCR (amastigotes/ml)a |
|---|---|---|---|---|---|---|
| IFN-γ producer cats | ||||||
| Serology- and PCR-negative | 25 (14) | 174 (64.2; 659.9) | 2280 (500.7; 4068) | 3.4 (2.1; 6.8) | 20 (0; 40) | 0 (0; 0) |
| Serology-positive and PCR-negative | 6 (3) | 193.9 (103.4; 685.3) | 4456 (1756; 5734) | 8.9 (1.4; 16.1) | 80 (70;140) | 0 (0; 0) |
| Serology- and PCR-positive | 1 (1) | 276.3 | 2083.9 | > 346.3 | 40960 | 125 |
| Apparently healthy | 21 (18) | 116 (63.7; 238.9) | 2097 (205.7; 3976) | 3.2 (1.4; 6.1) | 20 (0; 40) | 0 (0; 0) |
| Sick | 11 (18) | 567.1 (276.3; 1747) | 3509 (2326; 4967) | 6.9 (4.1; 12.7) | 20 (0; 80) | 0 (0; 0) |
| Total | 32 (18) | 203.5 (65.9; 586.7) | 2360 (979.1; 4297) | 3.7 (2.1; 8.1) | 20 (0; 40) | 0 (0; 0) |
| IFN-γ non-producer cats | ||||||
| Serology- and PCR-negative | 115 (64) | 0 (0; 0) | 646.1 (137.9; 2279) | 4.2 (2.4; 6.3) | 20 (0; 40) | 0 (0; 0) |
| Serology-positive and PCR-negative | 24 (13) | 0 (0; 0) | 803.2 (176; 2553) | 7.65 (4.7; 14.2) | 80 (50; 160) | 0 (0; 0) |
| Serology- and PCR-positive | 8 (4) | 0 (0; 0) | 1334 (303.8; 2370) | 25.5 (9.7; 263.5) | 320 (80; 1120) | 60 (7.5; 170) |
| Apparently healthy | 98 (82) | 0 (0; 0) | 587.5 (127.4; 2449) | 4.3 (2.6; 6.7) | 40 (0; 40) | 0 (0; 0) |
| Sick | 49 (82) | 0 (0; 0) | 1019 (204.7; 2219) | 5.8 (3.0; 8.9) | 20 (10; 40) | 0 (0; 0) |
| Total | 147 (82) | 0 (0; 0) | 742.5 (150.7; 2407) | 4.5 (2.8; 7.2) | 40 (0; 40) | 0 (0; 0) |
aMedian values (25th and 75th percentiles)