Fig. 2.

Logarithmic growth of LU-HNSCC-26. a. Cells in passage 14 were seeded in eight 96-well plates. The plates were analyzed by the sulphorhodamine b (SRB) method consecutively at the indicated time points. Error bars indicate standard error of the mean. The data points were fitted to a logarithmic equation, yielding a doubling time of 3.51 days = 84 (± 1.8) h. B. Tumors of LU-HNSCC-26 were retransplanted to 10 nude mice, two tumors per mouse. Tumors were measured with calipers three times per week and the relative tumor size calculated in relation to day 0, when all tumors were growing (except one that was excluded from analysis). Error bars indicate standard error of the mean. The data points were fitted to a logarithmic equation, yielding a doubling time (Td) of 7.38 days = 177 (± 27) h