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. 2019 Mar 19;10:150. doi: 10.3389/fendo.2019.00150

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Copyright © 2019 Kawabe, Waterson and Mizoguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose-dependent effects of bombyxin injection on the respiration rate of larvae. Three isolated abdomens were injected with bombyxin, and another three isolated abdomens were injected with vehicle, and their respiration was observed over 1 h to determine the respiration rate (μl/min). The average value for the respiration rates of bombyxin-injected larvae was divided by that of control larvae to calculate a relative respiration rate. The same test was repeated 4 times for each dose of bombyxin-II. The graph shows the means + SEM of the relative respiration rates for each dose of bombyxin-II. Student's t-test, **P < 0.01.