Figure 4.

The effects of bombyxin injection on the Fru-2,6-P₂ content in tissues. The isolated abdomens of day-3 fifth instar larvae were injected with 10 ng of bombyxin-II or vehicle, and various tissues were dissected 1 h after the injection to determine the Fru-2,6-P₂ content in each tissue. Only the wing discs were dissected from neck-ligated day-8 fifth instar larvae. In one experiment, 3–6 samples for each tissue were assayed to calculate an average Fru-2,6-P₂ amount relative to the control. The same experiment was repeated 2–5 times and the means + SEM of the relative Fru-2,6-P₂ amounts are shown in the graph. Student's t-test, *P < 0.05, **P < 0.01.