Figure 2.

THZ1 exhibited a powerful antitumor effect in cervical cancer cells.

Notes: (A) A CCK-8 assay was used to assess cell viability in cervical cancer cells after treatment with increasing concentrations of THZ1 at 24, 48, and 72 hours. Dose– response curves of cervical cancer cell lines after treatment with increasing concentrations of THZ1 at 24, 48, and 72 hours. Data are represented as the mean ± SD of three replicates. (B) Cell apoptosis was measured using a flow cytometry analysis of annexin V–fluorescein isothiocyanate/propidium iodide staining after treatment of THZ1 at the indicated concentrations in cervical cancer cells. (C) The proportion of annexin V-positive cells was measured in cervical cancer cell lines after treatment with THZ1 for 24 hours (*P<0.05, Student’s t-test). (D) Western blot was employed to assess cell apoptosis/necrosis proteins after THZ1 treatment for 24 hours.

Abbreviations: AV, annexin V; CCK-8, Cell Counting Kit-8; PARP, poly(ADP-ribose).