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. 2018 Nov 15;66(1):497–504. doi: 10.1111/tbed.13051

Figure 2.

Figure 2

Comparison of ‘wet’ and lyophilized reagents using direct detection by RT‐LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV‐1 sample NC‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted ½, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water (NFW) and compared to extracted RNA from the ‘neat’ sample as a positive control