Table 2.

Analytical sensitivity of the two SVV‐1 RT‐LAMP assays using either P1 or P2 and compared to the rRT‐PCR

Sample/test	Primer set	Dilution
		10−1	10−2	10−3	10−4	10−5	10−6	10−7	10−8	10−9
NC‐88‐23626
RT‐LAMP (T p)	P1	07:30	07:20	08:00	09:00	12:25	12:45a	12:30a	No T p	No T p
	P2	06:55	07:40	09:05	10:35	16:45a	No T p	No T p	No T p	No T p
rRT‐PCR (C T)		18.97	22.29	25.67	29.01	32.30	35.67	38.49	40.86a	Undet.
LA‐97‐1278
RT‐LAMP (T p)	P1	06:50	07:10	08:30	09:05	11:20	14:50	12:15a	No T p	No T p
	P2	08:05	09:10	10:50	13:35	No T p	No T p	No T p	No T p	No T p
rRT‐PCR (C T)		17.00	20.24	23.57	26.96	30.45	33.74	37.19	39.85b	Undet.

Values are means of three replicates. NC‐88‐23626 and LA‐97‐1278 are SVV‐1 isolates.

P1: primer set 1, P2: primer set 2.

^a C _T/T _p values for only one well.

^b C _T/T _p values for only two wells. Undet. C _T value undetermined by rRT‐PCR.