| Time interval (min) | Gradient (%B) | Flow rate (μl/min) |
|---|---|---|
| 0 | 0 | 350 |
| 0.1 | 4 | 350 |
| 32 | 12 | 350 |
| 60 | 22 | 350 |
| 70 | 30 | 350 |
| 75 | 70 | 350 |
| 80 | 70 | 350 |
| 100 | 0 | 350 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.